HSF1 Antibody

ProSci
Product Code: PSI-79-385
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-385-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from MCF7 cells using HSF1 (Ab-303).
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 (Ab-303).

Western blot analysis of lysed extracts from MCF7 cells using HSF1 (Ab-303).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 (Ab-303).

Further Information

Additional Names:
HSTF1, CMKBR7, EBI1, EVI1, HSTF 1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Background References:
  • Parvaneh Rafiee, et, al. (2006) Am J Physiol Cell Physiol ; 291: C931 - C945Fumika Shinozaki, et, al. (2006) J. Biol. Chem ; 281: 16361 - 16369. Eiichi Takaki, et, al. (2006) J. Biol. Chem ; 281: 4931 - 4937. Jan-Jong Hung, et, al. (1998) J. Biol. Chem ; 273: 31924.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
HSF1 (Ab-303) antibody was raised against a peptide sequence around aa.301~305 (P-P-S-P-P) derived from Human HSF1.
NCBI Gene ID #:
3297
NCBI Official Name:
heat shock transcription factor 1
NCBI Official Symbol:
HSF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
82 kDa
Protein Accession #:
NP_005517.1
Protein GI Number:
5031767
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total HSF1 H3.1 protein.
Swissprot #:
Q00613