IKK a Antibody

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ProSci
Product Code: PSI-79-328
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-328-0.1mg0.1mg£481.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HeLa, 293 and 3T3 cells using IKK α (Ab-23).
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK α (Ab-23).
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Western blot analysis of lysed extracts from HeLa, 293 and 3T3 cells using IKK α (Ab-23).
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK α (Ab-23).

Documents

Further Information

Additional Names:
IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, H2BFF, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Acts as part of the IKK complex in the conventional pathway of NF-κ-B activation and phosphorylates inhibitors of NF-κ-B thus leading to the dissociation of the inhibitor/NF-κ-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-κ-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-κ-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-κ-B-regulated promoters during inflammatory responses triggered by cytokines.
Background References:
  • Yuan ZQ, et al. (2002) J Biol Chem; 277 (33): 29973-82. Ozes ON, et al. (1999) Nature; 401 (6748): 82-5.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IKK a (Ab-23) antibody was raised against a peptide sequence around aa.21~25 (L-G-T-G-G) derived from Human IKK α.
NCBI Gene ID #:
1147
NCBI Official Name:
conserved helix-loop-helix ubiquitous kinase
NCBI Official Symbol:
CHUK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
NP_001269.3
Protein GI Number:
62241001
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of total IKKα protein.
Swissprot #:
O15111