LC3 antibody

ProSci
Product Code: PSI-79-626
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-626-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of extract from K-562, HeLa, JK and 293 cells using LC3 Antibody.

Western blot analysis of extract from K-562, HeLa, JK and 293 cells using LC3 Antibody.

Further Information

Additional Names:
LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3
Application Note:
Western Blot: 1:500~1:1000
Background:
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Background References:
  • He H., Dang Y., Dai F., Guo Z., Wu J., She X., Pei Y., Chen Y., Ling W., Wu C., Zhao S., Liu J. O., Yu L. J. Biol. Chem. 278:29278-29287 (2003) Tanida I., Sou Y. -S., Ezaki J., Minematsu-Ikeguchi N., Ueno T., Kominami E. J. Biol. Chem. 279:36268-36276 (2004)
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LC3 antibody was raised against a peptide sequence around aa. 112-116 (V-Y-A-S-Q) derived from Human LC3.
NCBI Gene ID #:
84557
NCBI Official Name:
microtubule-associated protein 1 light chain 3 alpha
NCBI Official Symbol:
MAP1LC3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
NP_115903.1
Protein GI Number:
14210522
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of total LC3 protein.
Swissprot #:
Q9H492