LIN28B Antibody
| Code | Size | Price |
|---|
| PSI-79-728-0.1mg | 0.1mg | £481.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
CSDD2, CTNNB, OK/SW-cl.35, PRO2286, CSDD2, FLJ16517
Application Note:
Western Blot: 1:500~1:1000
Background:
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Background References:
- "Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma. "Guo Y., Chen Y., Ito H., et al. Gene 384:51-61 (2006) "Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA. "Heo I., Joo C., Cho J., Ha M., Han J., Kim V. N. Mol. Cell 32:276-284 (2008) "TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation. "Heo I., Joo C., Kim Y. -K., Kim V. N., et al. Cell 138:696-708 (2009)
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LIN28B antibody was raised against a peptide sequence around aa.242~246 ( P-S-V-Q-K) derived from Human LIN28B.
NCBI Gene ID #:
389421
NCBI Official Name:
lin-28 homolog B (C. elegans)
NCBI Official Symbol:
LIN28B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 32 kDa
Protein Accession #:
NP_001004317.1
Protein GI Number:
51972214
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Stem Cell
SPECIFICITY:
This antibody detects endogenous levels of total LIN28B protein.
Swissprot #:
Q6ZN17