MKK3 Antibody

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ProSci
Product Code: PSI-79-325
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-325-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from MDA cells using MKK3 (Ab-189).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Ab-189).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MKK3 (Ab-189).
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Western blot analysis of lysed extracts from MDA cells using MKK3 (Ab-189).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Ab-189).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MKK3 (Ab-189).

Documents

Further Information

Additional Names:
MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2, ERK1, PRKM3, MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MEK3
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
MEK3 belongs to MAPKK family. This kinase is activated by mitogenic and environmental stress, and participates in the MAPK-mediated signaling cascade. It phosphorylates and thus activates p38. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of Ras oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of p38, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis.
Background References:
  • Wang W, et al. (2002) Mol Cell Biol ; 22 (10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16 (3): 1247-55.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MKK3 (Ab-189) antibody was raised against a peptide sequence around aa.187~191 (V-D-S-V-A) derived from Human MKK3.
NCBI Gene ID #:
5606
NCBI Official Name:
mitogen-activated protein kinase kinase 3
NCBI Official Symbol:
MAP2K3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
NP_002747.2
Protein GI Number:
21618347
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total MKK3 protein.
Swissprot #:
P46734