p62Dok Antibody

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ProSci
Product Code: PSI-79-393
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-393-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from K562, HL-60 and JK cells using p62Dok (Ab-398).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok (Ab-398).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using p62Dok (Ab-398).
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Western blot analysis of lysed extracts from K562, HL-60 and JK cells using p62Dok (Ab-398).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok (Ab-398).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using p62Dok (Ab-398).

Documents

Further Information

Additional Names:
P62DOK, CK2A1, DOK1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Background References:
  • Michael J. Wick, et, al. (2001) J. Biol. Chem; 276: 42843 - 42850. Paul D. Simoncic, et, al. (2006) Mol. Cell. Biol; 26: 4149 - 4160. Nadia Dube, et, al. (2004) PNAS; 101: 1834. Adam C. Yopp, et al. (2005) J. Immunol; 175: 2913 - 2924.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
p62Dok (Ab-398) antibody was raised against a peptide sequence around aa.396~400 (P-I-Y-D-E) derived from Human p62Dok.
NCBI Gene ID #:
1796
NCBI Official Name:
docking protein 1, 62kDa (downstream of tyrosine kinase 1)
NCBI Official Symbol:
DOK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
NP_001372.1
Protein GI Number:
4503357
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total p62Dok protein.
Swissprot #:
Q99704