Paxillin Antibody

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ProSci
Product Code: PSI-79-358
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-358-0.1mg0.1mg£481.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells using Paxillin (Ab-31).
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (Ab-31).
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Western blot analysis of lysed extracts from 293 cells using Paxillin (Ab-31).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (Ab-31).

Documents

Further Information

Additional Names:
PAXI, PXN
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions
Background References:
  • Davidson D, et al. (2001) EMBO J; 20 (13): 3414-3426Kook S, et al. (2000) Mol Biol Cell; 11 (3): 929-939Fleming I, et al. (1999) Proc Natl Acad Sci U S A; 96 (3): 1123-1128Goldberg MB, et al. (2001) Microbiol Mol Biol Rev; 65 (4): 595-626Thomas JT, et al. (1999) Proc Natl Acad Sci U S A; 96 (15): 8449-8454
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Paxillin (Ab-31) antibody was raised against a peptide sequence around aa.29~33 (T-P-Y-S-Y) derived from Human Paxillin.
NCBI Gene ID #:
5829
NCBI Official Name:
paxillin
NCBI Official Symbol:
PXN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
NP_001074324.1
Protein GI Number:
170932514
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total Paxillin protein.
Swissprot #:
P49023