Paxillin Antibody
| Code | Size | Price |
|---|
| PSI-79-470-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
NACP, PARK1, PAXI, PXN
Application Note:
Western Blot: 1:500~1:1000
Background:
An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions
Background References:
- Davidson D, et al. (2001) EMBO J 20 (13): 3414-3426. Fleming I, et al. (1999) Proc Natl Acad Sci U S A 96 (3): 1123-1128. Goldberg MB. (2001) Microbiol Mol Biol Rev 65 (4): 595-626. Kook S, et al. (2000) Mol Biol Cell 11 (3): 929-939.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Paxillin (Phospho-Tyr31) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 31 (T-P-Y (p) -S-Y) derived from Human Paxillin.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5829
NCBI Official Name:
paxillin
NCBI Official Symbol:
PXN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
NP_001074324.1
Protein GI Number:
170932514
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 31.
Swissprot #:
P49023