PI3K P85 Antibody
| Code | Size | Price |
|---|
| PSI-79-505-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
p85, AGM7, GRB1, p85-ALPHA, LHR, MDU2, MDU3, MIC4, PI3-kinase p85 subunit alpha, PtdIns-3-kinase p85-alpha
Application Note:
Western Blot: 1:500~1:1000
Background:
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1./Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
Background References:
- Stoyanov, B. et al. (1995) Science 269, 690-3. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41. Neri, L. M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PI3K P85 (phospho-Tyr467) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 467 (L-Y (p) -E-E-Y) derived from Human PI3K P85.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5295
NCBI Official Name:
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
NCBI Official Symbol:
PIK3R1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
NP_852556.2 / NP_001107644.1 / NP_005018.1
Protein GI Number:
32455252 / 166795247 / 4826908
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of total PI3K P85 only when phosphorylated at tyrosine 467.
Swissprot #:
P27986 Q92569 O00459