PKCdelta Antibody

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ProSci
Product Code: PSI-79-401
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-401-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from PC12 cells using PKCδ (Ab-645).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCδ (Ab-645).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using PKCδ (Ab-645).
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Western blot analysis of lysed extracts from PC12 cells using PKCδ (Ab-645).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCδ (Ab-645).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using PKCδ (Ab-645).

Documents

Further Information

Additional Names:
MAY1, PKCD, CVID9, nPKC-delta, G0S7
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol. which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
Background References:
  • Kei Sakamoto, et, al. (2003) Am J Physiol Endocrinol Metab, ; 285: E1081 - E1088. Ling Zhang, et, al. (2004) J. Biol. Chem ; 279: 28315 - 28319. Kristof Van Kolen et, al. (2006) FEBS J ; 273: 1843 - 1854. Martin Villalba, et, al. (2002) J. Cell Biol ; 157: 253.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PKCdelta (Ab-645) antibody was raised against a peptide sequence around aa. 643~647 (R-L-S-Y-S) derived from Human PKCδ.
NCBI Gene ID #:
5580
NCBI Official Name:
protein kinase C, delta
NCBI Official Symbol:
PRKCD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
78 kDa
Protein Accession #:
NP_006245.2
Protein GI Number:
31377782
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Apoptosis,Cancer,Neuroscience
SPECIFICITY:
This antibody detects endogenous level of total PKCδ protein.
Swissprot #:
Q05655