PLC-gamma1 Antibody

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ProSci
Product Code: PSI-79-506
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-506-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum using PLC-γ1 (phospho-Tyr771).
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Immunofluorescence staining of methanol-fixed HeLa cells using PLC-γ1 (phospho-Tyr771).
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Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum using PLC-γ1 (phospho-Tyr771).
Immunofluorescence staining of methanol-fixed HeLa cells using PLC-γ1 (phospho-Tyr771).

Documents

Further Information

Additional Names:
PLC1, NCKAP3, PLC-II, PLC148, PLCgamma1, CTNNB, OK/SW-cl.35, PRO2286, Phosphoinositide phospholipase C, Phospholipase C-gamma-1
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Background References:
  • Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027. Margolis, B. et al. (1989) Cell 57, 1101-1107. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PLC-gamma1 (phospho-Tyr771) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 771 (P-D-Y (p) -G-A) derived from Human PLC-γ1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5335
NCBI Official Name:
phospholipase C, gamma 1
NCBI Official Symbol:
PLCG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
155 kDa
Protein Accession #:
NP_002651.2
Protein GI Number:
33598948
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Neuroscience
SPECIFICITY:
This antibody detects endogenous level of PLC-gamma1 only when phosphorylated at tyrosine 771.
Swissprot #:
P19174