PLCgamma2 Antibody

Contact us
ProSci
Product Code: PSI-79-462
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-462-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

1 / 2
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using PLCγ2 (Phospho-Tyr753).
2 / 2
Immunofluorescence staining of methanol-fixed HeLa cells using PLCγ2 (Phospho-Tyr753).
❮❯

Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using PLCγ2 (Phospho-Tyr753).
Immunofluorescence staining of methanol-fixed HeLa cells using PLCγ2 (Phospho-Tyr753).

Documents

Further Information

Additional Names:
FCAS3, APLAID, PLC-IV, PLC-gamma-2, MEK6, MKK6, PRKMK6, SKK3, PLC-gamma2, Phospholipase C-gamma-2
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1, 4, 5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Background References:
  • Kim YJ, et al. (2004) Mol Cell Biol 24: 9986-9999Humphries LA, et al. (2004) J Biol Chem 279 : 37651-37661Suzuki-Inoue K, et al. (2004) Biochem J 378 : 1023-1029Rodriguez R, et al. (2003) Biochem J 374 : 269-280
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PLCgamma2 (Phospho-Tyr753) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 753 (S-L-Y (p) -D-V) derived from Human PLCγ2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5336
NCBI Official Name:
phospholipase C, gamma 2 (phosphatidylinositol-specific)
NCBI Official Symbol:
PLCG2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
150 kDa
Protein Accession #:
NP_002652.2
Protein GI Number:
117320537
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Neuroscience
SPECIFICITY:
This antibody detects endogenous level of PLCγ2 only when phosphorylated at tyrosine 753.
Swissprot #:
P16885