Rb Antibody
| Code | Size | Price |
|---|
| PSI-79-322-0.1mg | 0.1mg | £481.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
RB, pRb, OSRC, pp110, p105-Rb, CDC2, CDC28A, CDKN1, P34CDC2, P105-RB, PP105, PP110, RB-1, RB1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Rb operates in the midst of the cell cycle clock apparatus. Its main role is to act as a signal transducer connecting the cell cycle clock with the transcriptional machinery (Ref.1). It plays an important role in the Rb/ E2F pathway in cell proliferation, cell fate determination, and cancer (Ref.2). Cellular senescence is a stable form of cell cycle arrest that limits proliferation of damaged cells and act as a natural barrier to cancer progression. A distinct heterochromatic structure that accumulates in senescent human fibroblasts, designated as SAHF (Senescence-Associated Heterochromatic Foci). SAHF formation coincides with recruitment of heterochromatin proteins and the Rb protein to E2F-responsive promoters and is associated with the stable repression of E2F target genes. Both SAHF formation and the silencing of E2F target genes depended on the integrity of the Rb pathway and do not occur in reversibly arrested cells (Ref.3). Rb activates transcription of the c-Jun gene through the SP1-binding site within the c-Jun promoter
Background References:
- Roesch A, et al. (2005) Mod Pathol. 18 (4): 565-572. Chadee DN, et al. (2004) Nat Cell Biol. 6 (8): 770-776. Knudsen ES, et al. (1997) Mol Cell Biol. 17 (10): 5771-5783. Knudsen ES, et al. (1996) J Biol Chem. 271 (14): 8313-8320.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rb (Ab-807) antibody was raised against a peptide sequence around aa.805~809 (Y-I-S-P-L) derived from Human Rb.
NCBI Gene ID #:
5925
NCBI Official Name:
retinoblastoma 1
NCBI Official Symbol:
RB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
110 kDa
Protein Accession #:
NP_000312.2
Protein GI Number:
108773787
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Cell Cycle
SPECIFICITY:
This antibody detects endogenous level of total Rb protein.
Swissprot #:
P06400