Shc1 Antibody

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ProSci
Product Code: PSI-79-412
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-412-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HepG2 cells using Shc1 (Ab-427).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc1 (Ab-427).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Shc1 (Ab-427).
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Western blot analysis of lysed extracts from HepG2 cells using Shc1 (Ab-427).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc1 (Ab-427).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Shc1 (Ab-427).

Documents

Further Information

Additional Names:
SHC, SHCA, GLUH1, GLUR1, SH2 domain protein C1, SHC, SHC-transforming protein 1, Src homology 2 domain-containing-transforming protein C1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span
Background References:
  • Trampont P, et al. (2006) Mol Cell Biol; 26 (23): 9035-9044. Patrussi L, et al. (2005) Oncogene; 24 (13): 2218-2228van der Geer P, et al. (1996) Curr Biol ; 6 (11): 1435-1444
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Shc1 (Ab-427) antibody was raised against a peptide sequence around aa.425~429 (P-S-Y-V-N) derived from Human Shc1.
NCBI Gene ID #:
6464
NCBI Official Name:
SHC (Src homology 2 domain containing) transforming protein 1
NCBI Official Symbol:
SHC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46, 52, 66 kDa
Protein Accession #:
NP_001123512.1
Protein GI Number:
194239664
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total Shc1 protein.
Swissprot #:
P29353