syk Antibody

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ProSci
Product Code: PSI-79-434
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-434-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from JK cells using syk (Ab-323).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using syk (Ab-323).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using syk (Ab-323).
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Western blot analysis of lysed extracts from JK cells using syk (Ab-323).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using syk (Ab-323).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using syk (Ab-323).

Documents

Further Information

Additional Names:
p72-Syk, IKBE, Spleen tyrosine kinase
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C γdependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion
Background References:
  • Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447. Turner, M. et al. (2000) Immunol. Today 21, 148-154. Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874. Law, C. L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
syk (Ab-323) antibody was raised against a peptide sequence around aa. 321~325 (N-P-Y-E-P) derived from Human syk.
NCBI Gene ID #:
6850
NCBI Official Name:
spleen tyrosine kinase
NCBI Official Symbol:
SYK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
72 kDa
Protein Accession #:
NP_001128524.1
Protein GI Number:
205277435
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total syk protein.
Swissprot #:
P43405