TIF1beta Antibody
| Code | Size | Price |
|---|
| PSI-79-736-0.1mg | 0.1mg | £481.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
KAP1, TF1B, RNF96, TIF1B, KAP1
Application Note:
Western Blot: 1:500~1:1000
Background:
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.
Background References:
- Friedman J. R., Fredericks W. J., Jensen D. E., Speicher D. W., Huang X. -P., Neilson E. G., Rauscher F. J. IIIGenes Dev. 10:2067-2078 (1996) Moosmann P. R., Georgiev O., le Douarin B., Bourquin J. -P., Schaffner W. Nucleic Acids Res. 24:4859-4867 (1996) Agata Y., Matsuda E., Shimizu A. J. Biol. Chem. 274:16412-16422 (1999)
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TIF1beta antibody was raised against a peptide sequence around aa.827~831 (L-S-G-G-P) derived from Human TIF1β.
NCBI Gene ID #:
10155
NCBI Official Name:
tripartite motif containing 28
NCBI Official Symbol:
TRIM28
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
110 kDa
Protein Accession #:
NP_005753.1
Protein GI Number:
5032179
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Cell Cycle,Stem Cell
SPECIFICITY:
This antibody detects endogenous level of total TIF1β protein.
Swissprot #:
Q13263