Tyrosine Hydroxylase Antibody
| Code | Size | Price |
|---|
| PSI-79-611-0.1mg | 0.1mg | £481.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
TYH, DYT14, DYT5b, TYH, TH
Application Note:
Western Blot: 1:500~1:1000
Background:
Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms . This enzyme is required for embryonic development since TH knockout mice die before or at birth . Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH . Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels.
Background References:
- Kumer, S. C. and Vrana, K. E. (1996) J Neurochem 67, 443-62. Bodeau-Pean, S. et al. (1999) J Biol Chem 274, 3469-75. Kobayashi, K. et al. (1995) J Biol Chem 270, 27235-43. Lew, J. Y. et al. (1999) Mol Pharmacol 55, 202-9. Vie, A. et al. (1999) J Biol Chem 274, 16788-95. Lindgren, N. et al. (2000) J Neurochem 74, 2470-7. Moy, L. Y. and Tsai, L. H. (2004) J Biol Chem 279, 54487-93. Lehmann, I. T. et al. (2006) J Biol Chem 281, 17644-51. Saraf, A. et al. (2007) J Biol Chem 282, 573-80.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Tyrosine Hydroxylase (Ab-31) antibody was raised against a peptide sequence around aa.29~33 (V-T-S-P-R) derived from Mouse Tyrosine Hydroxylase.
NCBI Gene ID #:
7054
NCBI Official Name:
tyrosine hydroxylase
NCBI Official Symbol:
TH
NCBI Organism:
This antibody was raised against a peptide sequence around aa.29~33 (V-T-S-P-R) derived from Mouse Tyrosine Hydroxylase.
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 - 60 kDa
Protein Accession #:
NP_954986.2
Protein GI Number:
88900501
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Neuroscience
SPECIFICITY:
This antibody detects endogenous levels of total Tyrosine Hydroxylase protein.
Swissprot #:
P07101?