Zap-70 Antibody
| Code | Size | Price |
|---|
| PSI-79-347-0.1mg | 0.1mg | £481.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
SRK, STD, TZK, STCD, ZAP-70, NFKB3, 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the ζ chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-ζ chain, which can mediate the transduction of extracellular stimuli into cellular effector functions
Background References:
- Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100 (2): 443-448Ku GM, et al. (2001) EMBO J; 20 (3): 457-465Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96 (17): 9775-9780Zhao Q, et al. (1996) Mol Cell Biol; 16 (12): 6765-6774Williams BL, et al. (1999) EMBO J; 18 (7): 1832-1844Adjali O, et al. (2005) J Clin Invest; 115 (8): 2287-2295.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Zap-70 (Ab-319) antibody was raised against a peptide sequence around aa.317~321 (S-P-Y-S-D) derived from Human ZAP70.
NCBI Gene ID #:
7535
NCBI Official Name:
zeta-chain (TCR) associated protein kinase 70kDa
NCBI Official Symbol:
ZAP70
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
70 kDa
Protein Accession #:
NP_001070.2
Protein GI Number:
31455611
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total Zap-70 protein.
Swissprot #:
P43403