bNGF Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-38-239-0.5mg | 0.5mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
beta-NGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2
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Further Information
Additional Names:
Nerve growth factor, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428 , Beta-nerve growth factorBeta-NGF
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hB-NGF (10.0 ng/mL), a concentration of 2.7 - 4.0 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 200 pg/mL of recombinant human β-NGF when used with our biotinylated antigen affinity purified anti-human b-NGF as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hβ-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hB-NGF (10.0 ng/mL), a concentration of 2.7 - 4.0 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 200 pg/mL of recombinant human β-NGF when used with our biotinylated antigen affinity purified anti-human b-NGF as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hβ-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
beta-NGF antibody is produced in mice using highly pure (>98%) recombinant human beta-NGF as the immunizing antigen.
NCBI Gene ID #:
4803
NCBI Official Name:
nerve growth factor (beta polypeptide)
NCBI Official Symbol:
NGF
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01138
Protein GI Number:
90110037
Purification:
This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
Research Area:
Neuroscience, Functional Assays
Swissprot #:
P01138
User NOte:
Centrifuge vial prior to opening.