CCKBR Antibody

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ProSci
Product Code: PSI-45-378
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-378-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-378 (1ug/ml) staining of Mouse Pancreas (A) and Rat Stomach (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-378 Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.
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45-378 (1ug/ml) staining of Mouse Pancreas (A) and Rat Stomach (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-378 Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.

Documents

Further Information

Additional Names:
CCKBR, cholecystokinin B receptor , HGNC:1571, CCK-B, GASR, CCK2 receptor, cholecystokinin-B receptor/gastrin receptor, gastrin receptor, gastrincholecystokinin brain receptor, CCK2R
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80kDa band observed in Mouse Pancreas and Rat Stomach lysates (calculated MW of 49.2kDa according to Mouse NP_031653.1 and 49.0kDa according to Rat NP_037297.1). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 5ug/ml. In paraffin embedded Human Pancreas expression of CCKBR is detected in islet cells, and not in surrounding acinar cells. Recommended concentration: 6ug/ml.
Background References:
  • Olszewska-Pazdrak B, Townsend CM Jr, Hellmich MR. Agonist-independent activation of Src tyrosine kinase by a cholecystokinin-2 (CCK2) receptor splice variant. J Biol Chem. 2004 Sep 24;279(39):40400-4. Epub 2004 Jul 29.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-FDGDSDSDSQSRVRNQ
NCBI Gene ID #:
887
NCBI Official Name:
cholecystokinin B receptor
NCBI Official Symbol:
CCKBR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_795344.1
Protein GI Number:
28875799
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P32239