ChAT Antibody

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ProSci
Product Code: PSI-45-037
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-037-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-037 (1:2000) staining of PFA-perfused cryosection of Mouse Forebrain. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. ?va Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.
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45-037 (1:2000) staining of PFA-perfused cryosection of Mouse Forebrain. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. ?va Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.

Documents

Further Information

Additional Names:
Chat, choline acetyltransferase , B230380D24Rik
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3kDa according to Mouse NP_034021.1 and Rat NP_001164064.1). Recommended concentration:0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Frozen sections of Mouse Forebrain shows staining of neural cell bodies. Optimal dilution: 1:2000.
Background References:
  • Contestabile A, Fila T, Bartesaghi R, Contestabile A, Ciani E. Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases. J Neurochem. 2006 Apr;97(2):515-26. Epub 2006 Mar 15.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KERARGPTKPSNLD
NCBI Gene ID #:
12647 (mouse); 432359 (rat);
NCBI Official Name:
choline acetyltransferase
NCBI Official Symbol:
Chat
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_034021.1
Protein GI Number:
45476575
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience ,Immunology
SPECIFICITY:
This antibody is NOT expected to cross-react to Human CHAT.
Swissprot #:
Q03059