CHD5 Antibody

ProSci
Product Code: PSI-46-642
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-642-0.1mg0.1mg£565.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Further Information

Additional Names:
CHD5, chromodomain helicase DNA binding protein 5 , DKFZp434N231, KIAA0444
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 90kDa band in Human Brain (Cerebellum and Frontal Cortex) and Adrenal Gland lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 223kDa according to NP_056372.1. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Thompson PM, Gotoh T, Kok M, White PS, Brodeur GM. CHD5, a new member of the chromodomain gene family, is preferentially expressed in the nervous system. Oncogene. 2003 Feb 20;22(7):1002-11.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-QSRRQLKSDRDKP
NCBI Gene ID #:
26038
NCBI Official Name:
chromodomain helicase DNA binding protein 5
NCBI Official Symbol:
CHD5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056372.1
Protein GI Number:
24308089
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
Swissprot #:
Q8TDI0