COMT Antibody

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ProSci
Product Code: PSI-45-415
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-415-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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Staining of human testis lysate (35ug protein in RIPA buffer) with 45-415 (0.3ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.
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Staining of human testis lysate (35ug protein in RIPA buffer) with 45-415 (0.3ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
COMT, catechol-O-methyltransferase, OTTHUMP00000197749, OTTHUMP00000197750
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26kDa plus 29kDa bands observed in Human Testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and of 24.5kDa according to NP_009294.1). Recommended concentration: 0.3-1ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
  • Tunbridge EM, Harrison PJ, Weinberger DR. Catechol-o-Methyltransferase, Cognition, and Psychosis: Val(158)Met and Beyond. Biol Psychiatry. 2006 Feb 11;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: GDTKEQRILNHVLQC
NCBI Gene ID #:
1312
NCBI Official Name:
catechol-O-methyltransferase
NCBI Official Symbol:
COMT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000745.1, NP_001128633.1, NP_001128634.1, NP_009294.1
Protein GI Number:
4502969
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms. Variants (NP_000745.1; NP_001128633.1; NP_001128634.1) encode the same isoform.
Swissprot #:
P21964