DCX Antibody

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ProSci
Product Code: PSI-42-409
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-409-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-409 (0.01ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-409 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
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42-409 (0.01ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-409 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

Documents

Further Information

Additional Names:
DCX, doublecortin, RP5-914P14.1, DBCN, DC, FLJ51296, LISX, SCLH, XLIS, OTTHUMP00000023860, OTTHUMP00000216316, doublecortex, doublin, lis-X, lissencephalin-X, neuronal migration protein doublecortin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3kDa according to Mouse NP_001103692.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining. Recommended concentration: 2-4ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KTSANMKAPQS
NCBI Gene ID #:
1641
NCBI Official Name:
doublecortin
NCBI Official Symbol:
DCX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000546.2, NP_835365.1, NP_835364.1, NP_001182482.1
Protein GI Number:
30181246
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
Swissprot #:
O43602