DDB1 Antibody
| Code | Size | Price |
|---|
| PSI-45-048-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
DDB1, damage-specific DNA binding protein 1, 127kDa, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, DDB p127 subunit, damage-specific DNA binding protein 1 (127kD), XPE
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 140kDa band observed in lysates of cell line HeLa, HepG2 and Jurkat (calculated MW of 126.9kDa according to Human NP_001914.3). Recommended concentration: 1-2ug/ml.
Approx 140kDa band observed in lysates of cell line NIH3T3 and approx 150kDa band observed in lysates of cell line NSO (calculated MW of 126.8kDa according to Mouse NP_056550.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Kidney for this particular batch.
Background References:
- Dualan R, Brody T, Keeney S, Nichols AF, Admon A, Linn S. Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein. Genomics 1995 Sep 1;29(1):62-9
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DLIKVVEELTRIH
NCBI Gene ID #:
1642
NCBI Official Name:
damage-specific DNA binding protein 1, 127kDa
NCBI Official Symbol:
DDB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001914.3
Protein GI Number:
148529014
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
Q16531