Dysadherin Antibody

ProSci
Product Code: PSI-45-510
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-510-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-510 staining (0.5ug/ml) of Human Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

45-510 staining (0.5ug/ml) of Human Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
FXYD5, dysadherin, FXYD domain containing ion transport regulator 5, RIC, IWU1, IWU-1, dysad, HSPC113, KCT1, OIT2, PRO6241, FXYD domain-containing ion transport regulator 5, keratinocytes associated transmembrane protein 1, DYSAD, HSPC113
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 38kDa band observed in Human Spleen lysates (calculated MW of 19.5 kDa according to NP_054883 and NP_659003). The band was successfully blocked by incubation with the immunizing peptide. According to the literature this protein is O-glycosylated (PMID: 12672699), which may explain the size difference observed.
Background References:
  • Ino Y, Gotoh M, Sakamoto M, Tsukagoshi K, Hirohashi S. Dysadherin, a cancer-associated cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasis. Proc Natl Acad Sci USA. 2002 Jan 8;99(1):365-70.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: GKCRQLSRLCRNHCR
NCBI Gene ID #:
53827
NCBI Official Name:
FXYD domain containing ion transport regulator 5
NCBI Official Symbol:
FXYD5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_054883.3, NP_659003.1, NP_001158077.1
Protein GI Number:
7661784
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
NP_054883.3, NP_659003.1and NP_001158077.1 are variants that represent the same protein.
Swissprot #:
Q96DB9