FYN Antibody

ProSci
Product Code: PSI-45-059
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-059-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-059 (0.3ug/ml) staining of A431 (A) and Jurkat lysate (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45-059 (0.3ug/ml) staining of A431 (A) and Jurkat lysate (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
FYN, SLK, SYN, MGC45350, FYN oncogene related to SRC, FGR, YES, src-like kinase, c-syn protooncogene, tyrosine kinase p59fyn(T), src/yes-related novel gene, OKT3-induced calcium influx regulator, proto-oncogene tyrosine-protein kinase fyn, RP1-66H14.1, OTTHUMP00000017917, protein-tyrosine kinase fyn, src-like kinase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in lysates of cell lines A431 and Jurkat (calculated MW of 54.5kDa according to NP_694593.1). Recommended for use at 0.3-1ug/ml.
Background References:
  • Semba K, Nishizawa M, Miyajima N, Yoshida MC, Sukegawa J, Yamanashi Y, Sasaki M, Yamamoto T, Toyoshima K. yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family. Proc Natl Acad Sci U S A 1986 Aug;83(15):5459-63
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: VQCKDKEATKLTE
NCBI Gene ID #:
2534
NCBI Official Name:
FYN oncogene related to SRC, FGR, YES
NCBI Official Symbol:
FYN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002028.1, NP_694592.1, NP_694593.1
Protein GI Number:
4503823
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognise all three reported isoforms (as represented by NP_002028.1; NP_694592.1; NP_694593.1).
Swissprot #:
P06241