GAPDH Antibody

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ProSci
Product Code: PSI-45-060
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-060-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-060 (0.01ug/ml) staining of cell lines HEK293 (A) and HeLa (B) and Human Liver (C) and Tonsil (D) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-060 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
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45-060 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stai
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45-060 (0.01ug/ml) staining of cell lines HEK293 (A) and HeLa (B) and Human Liver (C) and Tonsil (D) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-060 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
45-060 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stai

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Further Information

Additional Names:
GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in lysates of cell lines Hek293 and HeLa and in Human Liver, Tonsil, Mouse Spleen, and Rat Brain and Spleen lysates (calculated MW of 36.1kDa according to Human NP_002037.2 and 35.8kDa according to Mouse NP_032110.1 and Rat NP_058704.1). Recommended concentration: 0.01-0.5ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 2.5ug/ml.Immunofluorescence: Expression of the protein seen in the cytoplasm and vesicles of HeLa cells. Recommended concentration: 10ug/ml.
Background References:
  • Burke JR, Enghild JJ, Martin ME, Jou YS, Myers RM, Roses AD, Vance JM, Strittmatter WJ. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nat Med. 1996 Mar;2(3):347-50.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: C-HQVVSSDFNSDT
NCBI Gene ID #:
2597
NCBI Official Name:
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol:
GAPDH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002037.2
Protein GI Number:
7669492
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis ReagentsControl Load Control
SPECIFICITY:
GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Swissprot #:
P04406