GATA3 Antibody

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ProSci
Product Code: PSI-45-062
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-062-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-062 (0.5ug/ml) staining of Human Tonsil (A) and Mouse Kidney (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
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45-062 (0.5ug/ml) staining of Human Tonsil (A) and Mouse Kidney (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Documents

Further Information

Additional Names:
GATA3, GATA binding protein 3, HDR, MGC2346, MGC5199, MGC5445, GATA-binding protein 3, enhancer-binding protein GATA3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 46kDa band observed in Human Tonsil and in Mouse Kidney lysates. (calculated MW of 47.9kDa according to NP_002042.1 and Mouse NP_032117.1 ). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Marine J, Winoto A. The human enhancer-binding protein Gata3 binds to several T-cell receptor regulatory elements. Proc Natl Acad Sci U S A 1991 Aug 15;88(16):7284-8
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: EVTADQPRWVSHH-C
NCBI Gene ID #:
2625
NCBI Official Name:
GATA binding protein 3
NCBI Official Symbol:
GATA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001002295.1, NP_002042.1
Protein GI Number:
50541959
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Immunology
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
Swissprot #:
P23771