GFAP Antibody

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ProSci
Product Code: PSI-45-063
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-063-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-063 (5ug/ml) as the reporter antibody.
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45-063 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
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45-063 (0.1ug/ml) staining of Mouse (A) and (0.01ug/ml) Rat (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-063 (5ug/ml) as the reporter antibody.
45-063 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-063 (0.1ug/ml) staining of Mouse (A) and (0.01ug/ml) Rat (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
GFAP, glial fibrillary acidic protein , FLJ45472 , intermediate filament protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa observed in Mouse and Rat Brain lysates (calculated MW of 49.9kDa according to Mouse NP_034407.2 and Rat NP_058705.2). Recommended concentration:0.01-0.1ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Korolainen MA, Auriola S, Nyman TA, Alafuzoff I, Pirttila T. Proteomic analysis of glial fibrillary acidic protein in Alzheimer's disease and aging brain. Neurobiol Dis. 2005 Dec;20(3):858-70. Epub 2005 Jun 27.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog
Immunogen:
The immunogen for this antibody is: C-DGEVIKESKQEHKD
NCBI Gene ID #:
2670
NCBI Official Name:
glial fibrillary acidic protein
NCBI Official Symbol:
GFAP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002046.1
Protein GI Number:
4503979
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.
Swissprot #:
P14136