GNB3 Antibody

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ProSci
Product Code: PSI-42-684
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-684-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-684 (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-684 (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
GNB3, guanine nucleotide binding protein (G protein), beta polypeptide 3, G protein, beta-3 subunit, GTP-binding regulatory protein beta-3 chain, guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3, guanine nucleotide-binding protein, beta-3 subunit, hypertension associated protein, transducin beta chain 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 35kDa band observed in lysates of cell line HepG2 (calculated MW of 37.2kDa according to NP_002066.1. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebellum shows strong axonal staining in the Molecular layer and less so in the Granular layer. Recommended concentration, 3-5ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: SGHDNRVSCLGVT
NCBI Gene ID #:
2784; 59345
NCBI Official Name:
guanine nucleotide binding protein (G protein), beta polypeptide 3
NCBI Official Symbol:
GNB3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002066.1, NP_067642.1
Protein GI Number:
4504053
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Obesity
SPECIFICITY:
This antibody is expected to recognize GNB3 & GNB4 (GeneID: 2784, 59345; ProteinID: NP_002066.1; NP_067642.1).
Swissprot #:
P16520