HPGD Antibody

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ProSci
Product Code: PSI-45-070
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-070-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-070 (0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-070 (0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
HPGD, Prostaglandin dehydrogenase 1, PGDH1, 15-PGDH, hydroxyprostaglandin dehydrogenase 15-(NAD), PGDH, SDR36C1, NAD+-dependent 15-hydroxyprostaglandin dehydrogenase, short chain dehydrogenase/reductase family 36C, member 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Experiments gave bands at approx 26kDa and 50kDa in Human Duodenum lysates after 0.1ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (PMID: 11889207). This protein has a calculated MW of 29.0kDa according to NP_000851. Recommended concentration: 0.1-1ug/ml.Immunohistochemistry:Paraffin embedded Human Colon and Liver. Recommended concentration: 2.5?g/ml.
Background References:
  • Coggins KG, Latour A, Nguyen MS, Audoly L, Coffman TM, Koller BH. Metabolism of PGE2 by prostaglandin dehydrogenase is essential for remodeling the ductus arteriosus. Nat Med. 2002 Feb;8(2):91-2. No abstract available.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DYDTTPFQAKTQ
NCBI Gene ID #:
3248
NCBI Official Name:
hydroxyprostaglandin dehydrogenase 15-(NAD)
NCBI Official Symbol:
HPGD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000851.2
Protein GI Number:
31542939
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P15428