HSD3B1 Antibody

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ProSci
Product Code: PSI-45-749
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-749-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-749 (0.01ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-749 (0.01ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
HSD3B1, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, HGNC:5217, HSD3B, HSDB3, Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in Human Placenta and Adrenal Gland lysates (calculated MW of 42.3kDa according to NP_000853.1). Recommended concentration: 0.003-0.01ug/ml.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.
Background References:
  • Chang BL, Zheng SL, Hawkins GA, Isaacs SD, Wiley KE, Turner A, Carpten JD, Bleecker ER, Walsh PC, Trent JM, Meyers DA, Isaacs WB, Xu J. Joint effect of HSD3B1 and HSD3B2 genes is associated with hereditary and sporadic prostate cancer susceptibility. Cancer Res. 2002 Mar 15;62(6):1784-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DRHKETLKSKTQ
NCBI Gene ID #:
3283
NCBI Official Name:
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
NCBI Official Symbol:
HSD3B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000853.1
Protein GI Number:
4504507
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P14060