HSP90B1 Antibody

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ProSci
Product Code: PSI-45-071
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-071-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-071 (0.1ug/ml) staining of U2OS (A), HEK293 (B), HeLa (C) and (0.3ug/ml) A549 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminecence.
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45-071 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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45-071 (0.1ug/ml) staining of U2OS (A), HEK293 (B), HeLa (C) and (0.3ug/ml) A549 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminecence.
45-071 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Documents

Further Information

Additional Names:
TRA1, GRP94, tumor rejection antigen (gp96) 1 , HGNC:12028, ECGP, GP96, Tumor rejection antigen-1 (gp96), endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, tumor rejection antigen (gp96) 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in lysates of cell lines HEK293, A549, HeLa, U2OS and NIH3T3 and in preliminary testing of MCF7 and HepG2 cell lysate (calculated MW of 92.5kDa according to Human NP_003290.1 and Mouse NP_035761.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3?g/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Uterus and Liver. Recommended concentration: 3-5?g/ml. Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of A431 and HeLa cells. Recommended concentration: 10?g/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Background References:
  • Stojkovic M, Lako M, Stojkovic P, Stewart R, Przyborski S, Armstrong L, Evans J, Herbert M, Hyslop L, Ahmad S, Murdoch A, Strachan T. Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture. Stem Cells. 2004;22(5):790-7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow, Zebrafish
Immunogen:
The immunogen for this antibody is: C-KEGVKFDESEKTKE
NCBI Gene ID #:
7184
NCBI Official Name:
heat shock protein 90kDa beta (Grp94), member 1
NCBI Official Symbol:
HSP90B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003290.1
Protein GI Number:
4507677
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
.
Swissprot #:
P14625