HTR7 Antibody
| Code | Size | Price |
|---|
| PSI-42-157-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
HTR7, 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled), 5-HT7, 5-hydroxytryptamine receptor 7, OTTHUMP00000020077, OTTHUMP00000020078, OTTHUMP00000020079, serotonin 5-HT-7 receptor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa band observed in lysates of cell line KELLY (calculated MW of 49.5kDa according to NP_000863.1). Recommended concentration: 1-3ug/ml.
An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- Sagren B, Svenningsson P, Depletion of the lipid raft constituents, sphingomyelin and ganglioside, decreases serotonin binding at human 5-HT7(a) receptors in HeLa cells. Acta physiologica (Oxford, England) 2007 May 190 (1): 47-53.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: QNADYCRKKGHDS
NCBI Gene ID #:
3363
NCBI Official Name:
5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled
NCBI Official Symbol:
HTR7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000863.1
Protein GI Number:
4504547
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience ,Signal Transduction
SPECIFICITY:
This antibody is expected to recognize isoform a (NP_000863.1) only.
Swissprot #:
P34969