Ide Antibody

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ProSci
Product Code: PSI-45-760
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-760-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-760 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-760 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
Ide, insulin-degrading enzyme, 1300012G03Rik, 4833415K22Rik, AA675336, AI507533, INSULYSIN, insulinase, insulysin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 118kDa according to NP_112419.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Zhao Z, Xiang Z, Haroutunian V, Buxbaum JD, Stetka B, Pasinetti GM. Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease. Neurobiol Aging. 2007 Jun;28(6):824-30. Epub 2006 Jun 12.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QQYNYDRDNIE
NCBI Gene ID #:
15925 (mouse); 25700 (rat);
NCBI Official Name:
insulin degrading enzyme
NCBI Official Symbol:
Ide
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_112419.2
Protein GI Number:
121583922
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9JHR7