IGF1 Antibody

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ProSci
Product Code: PSI-42-659
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-659-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-659 (0.5ug/ml) staining of Human Uterus (A) and negative control HepG2 (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
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42-659 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
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42-659 (0.5ug/ml) staining of Human Uterus (A) and negative control HepG2 (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
42-659 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Documents

Further Information

Additional Names:
IGF1, insulin-like growth factor 1 (somatomedin C), IGF1A, IGFI, OTTHUMP00000195084, insulin-like growth factor 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 15kDa band observed in Human Uterus lysates (calculated MW of 15.2kDa according to NP_001104754.1). Recommended concentration 0.5-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: HepG2 cell lysate. Preliminary testing was unsuccessful on?Mouse and Rat?for this particular batch.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-RSVRAQRHTD
NCBI Gene ID #:
3479
NCBI Official Name:
insulin-like growth factor 1 (somatomedin C)
NCBI Official Symbol:
IGF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001104753.1, NP_001104754.1, NP_001104755.1, NP_000609.1
Protein GI Number:
163659899
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1).
Swissprot #:
P05019