KEAP1 Antibody

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ProSci
Product Code: PSI-42-391
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-391-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-391 (0.2ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer).. Detected by chemiluminescence.
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42-391 (0.2ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer).. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
KEAP1, kelch-like ECH-associated protein 1, INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454, cytosolic inhibitor of Nrf2, kelch-like protein 19
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 69.7kDa according to Human NP_036421.2 and of 69.6kDa according to Mouse NP_057888.1). Recommended concentration: 0.2-0.6ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebellum shows textured cytoplasm staining in Purkinje cell and processes. Recommended concentration, 3-5ug/ml.Immunofluorescence:?Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-EVTPSQHGNRTFS
NCBI Gene ID #:
9817
NCBI Official Name:
kelch-like ECH-associated protein 1
NCBI Official Symbol:
KEAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036421.2
Protein GI Number:
22027642
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
Reported variants represent identical protein: NP_036421.2, NP_987096.1
Swissprot #:
Q14145