KIP1 Antibody
| Code | Size | Price |
|---|
| PSI-45-800-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
CDKN1B, KIP1, cyclin-dependent kinase inhibitor 1B (p27, Kip1) , CDKN4, P27KIP1, cyclin-dependent kinase inhibitor 1B
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2 (calculated MW of 22.1kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14028-33; PMID: 16169901). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Recommended concentration, 5-10ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
- Baldassarre G, Belletti B, Nicoloso MS, Schiappacassi M, Vecchione A, Spessotto P, Morrione A, Canzonieri V, Colombatti A. p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion. Cancer Cell. 2005 Jan;7(1):51-63.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KRPATDDSSTQNK
NCBI Gene ID #:
1027
NCBI Official Name:
cyclin-dependent kinase inhibitor 1B (p27, Kip1)
NCBI Official Symbol:
CDKN1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004055.1
Protein GI Number:
4757962
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P46527