LASS1 Antibody
| Code | Size | Price |
|---|
| PSI-46-947-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
LAG1, CerS1, LASS1, LAG1 homolog, ceramide synthase 1, MGC90349, UOG1, LAG1 longevity assurance homolog 1, longevity assurance (LAG1, S. cerevisiae) homolog 1, longevity assurance gene 1, upstream of GDF1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 37 kDa band observed in Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates (calculated MW of 38.0 kDa according to NP_937850.1). Recommended concentration: 1-3ug/ml.
An additional band of unknown identity was also consistently observed at 85 kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- Venkataraman K, Riebeling C, Bodennec J, Riezman H, Allegood JC, Sullards MC, Merrill AH Jr, Futerman AH. Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cells. J Biol Chem. 2002 Sep 20;277(38):35642-9. Epub 2002 Jun 24.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KDLREYDTAEAQ
NCBI Gene ID #:
10715
NCBI Official Name:
ceramide synthase 1
NCBI Official Symbol:
LASS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_067090.1, NP_937850.1
Protein GI Number:
11641421
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_067090.1and NP_937850.1).
Swissprot #:
P27544