MEL18 Antibody

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ProSci
Product Code: PSI-45-106
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-106-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-106 staining (1ug/ml) of A549 (A) and PD19 (B) cell lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
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45-106 staining (1ug/ml) of A549 (A) and PD19 (B) cell lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PCGF2, MEL-18, polycomb group ring finger 2, RNF110, ZNF144, ring finger protein 110, zinc finger protein 144, MGC10545
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37-38 kDa band observed in lysates of cell lines A549 and PD19 (calculated MW of 37.8kDa according to NP_009075.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Ishida A, Asano H, Hasegawa M, Koseki H, Ono T, Yoshida MC, Taniguchi M, Kanno M. Cloning and chromosome mapping of the human Mel-18 gene which encodes a DNA-binding protein with a new 'RING-finger' motif. Gene. 1993 Jul 30;129(2):249-55.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KMTVNGAPVPPLT
NCBI Gene ID #:
7703
NCBI Official Name:
polycomb group ring finger 2
NCBI Official Symbol:
PCGF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_009075.1
Protein GI Number:
6005964
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
P35227