MYD88 Antibody

ProSci
Product Code: PSI-45-095
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-095-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-095 staining (0.2ug/ml) of Human Thymus lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
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45-095 staining (0.2ug/ml) of Human Thymus lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

Further Information

Additional Names:
MYD88, myeloid differentiation primary response gene (88), MYD88D, myeloid differentiation primary response gene 88
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 33kDa band observed in Human Thymus lysates (calculated MW of 33.2kDa according to NP_002459.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:In paraffin embedded Human Tonsil shows staining of clusters of cells some of which are likely plasma cells. Recommended concentration: 4-6ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant MYD88 captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml.
Background References:
  • Li X, Qin J. Modulation of Toll-interleukin 1 receptor mediated signaling. J Mol Med. 2005 Jan 21;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-IKYKAMKKEFP
NCBI Gene ID #:
4615
NCBI Official Name:
myeloid differentiation primary response 88
NCBI Official Symbol:
MYD88
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001166038.1, NP_002459.2, NP_001166039.1
Protein GI Number:
4505297
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Innate Immunity
SPECIFICITY:
This antibody is expected to recognize reported isoforms 1, 2 and 3 (NP_001166038.1; NP_002459.2; NP_001166039.1).
Swissprot #:
Q99836