NCE2 Antibody

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ProSci
Product Code: PSI-42-142
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-142-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-142 (0.05ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-142 (0.05ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
UBE2F, ubiquitin-conjugating enzyme E2F (putative), MGC18120, NCE2, NEDD8 conjugating enzyme, NEDD8-conjugating enzyme
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 20kDa band observed in Mouse Liver lysates (calculated MW of 21.1kDa according to Human NP_542409.1 and to Mouse NP_080730.1). Recommended concentration: 0.05-0.2ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows membrane staining in trophoblasts-. Recommended concentration, 2.5-3.8ug/ml.
Background References:
  • Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol. Cell 33 (4), 483-495 (2009).
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HLRDKEDFRNKVDD
NCBI Gene ID #:
140739
NCBI Official Name:
ubiquitin-conjugating enzyme E2F (putative)
NCBI Official Symbol:
UBE2F
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_542409.1
Protein GI Number:
18087857
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q969M7