NCF1 Antibody

Product Code: PSI-45-096

Supplier: ProSci

Code	Size	Price

PSI-45-096-0.1mg

0.1mg

£626.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Human

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Western Blot (WB)

Storage:

Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-096 (0.2ug/ml) staining of U937 (A), (0.01ug/ml) Daudi (B), (0.3ug/ml) U251 (C) and negative control A431 (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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45-096 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

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45-096 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

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Documents

Further Information

Additional Names:

NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2

Application Note:

Peptide ELISA: antibody detection limit dilution 1:20000.Western Blot:Approx 48kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50kDa in Pig Spleen lysates (calculated MW of 44.7kDa according to Human NP_000256.4, 46.0kDa according to Mouse NP_001272966.1 and 45.3kDa according to Pig NP_001106691.1). The observed molecular weights correspond to different antibodies from other commercial sources. Recommended concentration: 0.01-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate. Preliminary testing was unsuccessful on Rat Spleen and Thymus for this particular batch.Immunohistochemistry:Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Background References:

Davidson-Moncada JK, Lopez-Lluch G, Segal AW, Dekker LV Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils. Biochem J 2002 Apr 1;363(Pt 1):95-103

Buffer:

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.

Concentration:

500 ug/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Expected Species Reactivity based on sequence homology: Rat, Cow

Immunogen:

The immunogen for this antibody is: C-SESTKRKLASAV

NCBI Gene ID #:

653361

NCBI Official Name:

neutrophil cytosolic factor 1

NCBI Official Symbol:

NCF1

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

NP_000256.4

Protein GI Number:

115298672

Purification:

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Research Area:

Homeostasis

SPECIFICITY:

Swissprot #:

P14598