Osteopontin Antibody

ProSci
Product Code: PSI-45-152
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-152-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-152 (0.1ug/ml) staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-152 (0.1ug/ml) staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
SPP1, HGNC:11255, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), BNSP, BSPI, ETA-1, MGC110940, OPN, Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), secreted phosphoprotein 1, SPP1/CALPHA1 fusion, bone sialoprotein I, early T-lymphocyte activation 1, osteopontin, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in lysates of cell line MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Kolb A, Kleeff J, Guweidhi A, Esposito I, Giese NA, Adwan H, Giese T, Buchler MW, Berger MR, Friess H. Osteopontin Influences the Invasiveness of Pancreatic Cancer Cells and is Increased in Neoplastic and Inflammatory Conditions. Cancer Biol Ther. 2005 Jul 5;4(7)
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DPKSKEEDKHLK
NCBI Gene ID #:
6696
NCBI Official Name:
secreted phosphoprotein 1
NCBI Official Symbol:
SPP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001035147.1, NP_000573.1, NP_001035149.1
Protein GI Number:
4759166
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
P10451