p70 S6 Kinase Antibody

Contact us
ProSci
Product Code: PSI-45-138
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-138-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 4
45-138 staining (0.05ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
2 / 4
3 / 4
4 / 4
45-138 (0.1ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
❮❯

45-138 staining (0.05ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
45-138 (0.1ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
RPS6KB1, p70-S6K, ribosomal protein S6 kinase, 70kD, polypeptide 1, PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-alpha, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, ribosomal protein S6 kinase, 70kD, polypeptide 1, serine/threonine kinase 14 alpha
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 65kDa band observed in lysates of Human Placenta, of Rat Brain and of cell line NIH3T3 (calculated MW of 59.1kDa according to NP_003152.1). Recommended concentration: 0.05-0.1ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows staining in the cytoplasm-rich PCT while less so in the DCT and no signals in the glomerulus. Recommended concentration: 2-4ug/ml.
Background References:
  • Grove JR, Banerjee P, Balasubramanyam A, Coffer PJ, Price DJ, Avruch J, Woodgett JR. Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini. Mol Cell Biol. 1991 Nov;11(11):5541-50.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-MISKRPEHLRMNL
NCBI Gene ID #:
6198
NCBI Official Name:
ribosomal protein S6 kinase, 70kDa, polypeptide 1
NCBI Official Symbol:
RPS6KB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003152.1
Protein GI Number:
4506737
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
P23443