PAFAH1B1 Antibody

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ProSci
Product Code: PSI-45-104
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-104-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-104 (0.1ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-104 (3.75ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
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45-104 (0.1ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-104 (3.75ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

Documents

Further Information

Additional Names:
PAFAH1B1, LIS1, platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa, MDCR, PAFAH, lissencephaly 1 protein, Platelet-activating factor acetylhydrolase, isoform 1B, alpha subunit, platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD), platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa), LIS2, MDS,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in Human Ovary and in Rat Ovary lysates (calculated MW of 46.6kDa according to Human NP_000421.1 and to Rat NP_113951.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cortex), Thyroid and Pancreas. Recommended concentration: 3.75ug/ml.
Background References:
  • Reiner O, Bar-Am I, Sapir T, Shmueli O, Carrozzo R, Lindsay EA, Baldini A, Ledbetter DH, Cahana A. LIS2, gene and pseudogene, homologous to LIS1 (lissencephaly 1), located on the short and long arms of chromosome 2. Genomics. 1995 Nov 20;30(2):251-6.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: TGSVDQTVKVWECR
NCBI Gene ID #:
5048
NCBI Official Name:
platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
NCBI Official Symbol:
PAFAH1B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000421.1
Protein GI Number:
4557741
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
.
Swissprot #:
P43034