PPP2C Antibody

ProSci
Product Code: PSI-45-119
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-119-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-119 (0.05ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-119 (0.05ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PPP2CA, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform, PP2Ac, PP2CA, RP-C, PP2A-alpha, protein phosphatase 2, catalytic subunit, alpha isoform, replication protein C, serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PPP2CB, protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform, PP2CB, PP2A-beta, protein phosphatase 2, catalytic subunit, beta isoform, protein phosphatase type 2A catalytic subunit, serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 36kDa band observed in Human Brain (Cerebellum), Mouse, Rat and Pig Brain lysates (calculated MW of 35.6kDa according to Human NP_002706.1, Mouse NP_062284.1, Rat NP_058735.1 and Pig NP_999531.1). Recommended concentration: 0.05-0.2ug/ml.
Background References:
  • Hemmings BA, Wernet W, Mayer R, Maurer F, Hofsteenge J, Stone SR. The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A beta catalytic subunit. Nucleic Acids Res 1988 Dec 9;16(23):11366
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-EPHVTRRTPDYFL
NCBI Gene ID #:
5515; 5516
NCBI Official Name:
protein phosphatase 2, catalytic subunit, alpha isozyme
NCBI Official Symbol:
PPP2CA / PPP2CB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002706.1, NP_004147.1
Protein GI Number:
4506017
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
Two variants NP_001009552.1 and NP_004147.1 represent identical protein.
Swissprot #:
P67775