PPP2R1A Antibody

ProSci
Product Code: PSI-45-120
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-120-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-120 (2ug/ml) staining of Human Thymus (A) and Human Lymph node (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-120 (2ug/ml) staining of Human Thymus (A) and Human Lymph node (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PPP2R1A, MGC786, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform, PP2A, subunit A, R1-alpha isoform, PP2A, subunit A, PR65-alpha isoform, medium tumor antigen-associated 61 KDA protein, Serine/threonine protein phosphatase 2A, 65 KDA regulatory subunit A, alpha isoform, protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform, PR65A, alpha isoform of regulatory subunit A, protein phosphatase 2, medium tumor antigen-associated 61 KDA protein, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 60-65kDa band observed in Human Thymus and Human Lymph node lysates (calculated MW of 65.3kDa according to NP_055040.2). Recommended concentration: 2-6ug/ml.
Background References:
  • Hong Y, Sarge KD. Regulation of protein phosphatase 2A activity by heat shock transcription factor 2. J Biol Chem. 1999 May 7;274(19):12967-70.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KYFAQEALTVLSLA
NCBI Gene ID #:
5518
NCBI Official Name:
protein phosphatase 2, regulatory subunit A, alpha
NCBI Official Symbol:
PPP2R1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055040.2
Protein GI Number:
21361399
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
P30153