Resistin Antibody

ProSci
Product Code: PSI-XP-5261
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5261-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Resistin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated free

Images

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To detect hResistin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human Resistin (XP-5261Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hResistin.
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To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

To detect hResistin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human Resistin (XP-5261Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hResistin.
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

Further Information

Additional Names:
ADSF, RSTN, XCP1, FIZZ3, RETN1, HXCP1, UNQ407/PRO1199, Resistin, Adipose tissue-specific secretory factor, ADSF
Application Note:
ELISA:

To detect hResistin by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hResistin.

Sandwich:

To detect hResistin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Resistin (XP-5261Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hResistin.

Western Blot:

To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Resistin is a cytokine that is specifically secreted by adipocytes and seems to suppress the ability of insulin to stimulate glucose uptake into adipose cells. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hResistin (human Resistin).
NCBI Gene ID #:
56729
NCBI Official Name:
resistin
NCBI Official Symbol:
RETN
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q9HD89
Protein GI Number:
18202962
Purification:
Anti-hResistin specific antibody was purified by affinity chromatography employing immobilized hResistin matrix.
Research Area:
Chemokines & Cytokines,Signal Transduction,Antibody Pairs
Swissprot #:
Q9HD89
User NOte:
Centrifuge vial prior to opening.